Cyprus Journal of Medical Sciences
Original Article
Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures

Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures

1.

Department of Medical Biochemistry, Ankara Yıldırım Beyazıt University School of Medicine, Ankara, Turkey

2.

Department of Biophysics, Adnan Menderes University School of Medicine, Aydın, Turkey

3.

Department of Medical Biology, Ankara Yıldırım Beyazıt University School of Medicine, Ankara, Turkey

4.

Department of Physics, Adnan Menderes University Faculty of Science and Art, Aydın, Turkey

5.

University of Bristol, School of Cellular and Molecular Medicine, Bristol, UK

Cyprus J Med Sci 2019; 4: 201-207
DOI: 10.5152/cjms.2019.804
Read: 203 Downloads: 101 Published: 23 December 2019

BACKGROUND/AIMS

Tissue engineering approaches have provided treatment options for patients with limited cartilage repair capacity. Most of these approaches rely on isolating and expanding chondrocytes in vitro. Mechanical stress, ultrasound, and electric and electromagnetic fields (EMFs) can be used to stimulate cartilage repair. EMF has been used in the management of conditions such as arthritis and fractures. Most of the previous studies have focused on low-frequency pulsed EMF (PEMF). The aim of the present study was to investigate the effects of low-frequency continuous (sinusoidal) EMF (CEMF) versus PEMF on chondrocytes.

 

MATERIAL and METHODS

Chondrocytes from bovine nasal cartilage were exposed to low-frequency CEMF versus PEMF, and the proliferation and differentiation capacities of these chondrocytes were determined. The effects of EMFs on retinoic acid receptor beta and transforming growth factor beta (TGF-β) expressions were investigated using quantitative reverse transcription polymerase chain reaction.

 

RESULTS

Our observations suggested that there was no difference between the effects of PEMF and CEMF exposure on the proliferation and differentiation capacities of chondrocytes.

 

CONCLUSION

EMF-mediated proliferation of chondrocytes requires the presence of growth factors, especially insulin-like growth factor, in the environment to maintain the chondrogenic phenotype; furthermore, the EMF effect on chondrocytes is independent of TGF-β.

 

Cite this article as: Kozacı LD, Bilgin MD, Çarhan A, Çalışkan ŞG, Kefienah W. Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. Cyprus J Med Sci 2019; 4(3): 201-7.

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ISSN2149-7893 EISSN 2536-507X